Whole Genome Characterization of Prunus Necrotic Ringspot Virus and Prune Dwarf Virus Infecting Stone Fruits in Russia

We conducted a survey of the phytosanitary status Prunus germplasm collection in Nikita Botanical Gardens, Yalta, Russia. The virome plants displaying virus-like symptoms was studied using Illumina MiSeq high-throughput sequencing. Reads related to prunus necrotic ringspot virus (PNRSV), prune dwarf (PDV), and ourmia-like 1 (OuLV1) were generated number samples. Near complete genomes two divergent PNRSV isolates, PDV isolate, contig partly covered OuLV1 genome assembled de novo metaSPAdes program. structure genomic RNA1, RNA2, RNA3 new ilarvirus isolates shown be typical PDV. This is first report characterization full-length from Russia, expanding information on their geographical distribution genetic diversity. An open reading frames (ORF)-based phylogeny all available GenBank divided each ORF into or three main clusters. A migrated one cluster another cluster, depending analyzed segment. different branching order may indicate reassortment evolutionary history some isolates.